Human Umbilical Vein Endothelial Cells Secrete Transcobalamin
نویسندگان
چکیده
Transcobalamin II (TC Ill is essential for cellular uptake of cobalamin. However, t h e origin of this t ransport protein is controversial and many organ sources have been suggested. We studied human umbilical vein endothelial cells cultured in vitro. The cells contained T C II (2.3 pmol/108 cells) and released progressively increasing amounts of t h e protein into t h e surrounding medium during t h e 3-day incubation period. This release exceeded t h e start ing intracellular content of TC II. In contrast , endothelial cells did not contain o r elaborate R binder, t h e o the r major circulating binding protein for cobalamin. Cycloheximide inhibited t h e elaboration of TC II, suggesting tha t t h e
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